Isolation and Preliminary Characterization of Two Glycophorins from Rabbit Erythrocyte Membranes

概要

論文の詳細を見る
Two different glycophorins were isolated from rabbit erythrocyte membranes. Crude glycophorin fraction prepared by extraction with lithium diiodosalicylate and partition in aqueous phenol contained water-soluble glycolipid in addition to glycophorins. After removal of the glycolipid from glycophorins by ion-exchange chromatography, glycophorins were effectively fractionated by two different techniques : gel chromatography on Bio-Gel A 1.5m and ion-exchange chromatography in the presence of a nonionic detergent, Ammonyx LO. Two glycophorins were isolated in a ratio of about 1 : 2 (10 mg and 18 mg/3 g lyophilized erythrocyte membranes). The minor component, designated glycophorin RA, was a sialoglycoprotein (42% protein and 58% carbohydrate) and the major one, designated glycophorin RB, was of smaller size and lower carbohydrate content (57% protein and 43% carbohydrate). Glycophorins RA and RB were also different in chemical composition and amino-terminal sequence. Further evidence that the structures of the two glycophorins are unrelated to each other was obtained by Ouchterlony immunodiffusion with lectins ; glycophorin RB precipitated with concanavalin A and Ricinus communis agglutinin, whereas glycophorin RA did not.
社団法人日本薬学会の論文
1982-03-25