Studies on Biotransformation of Lysozyme. I. Preparation of Labeled Lysozyme and Its Intestinal Absorption

概要

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The preparation and stability of lysozyme labeled with ^3H or ^<131>I were studied. ^<131>I-lysozyme was found to be convenient for investigation on biotransformation of the enzyme. The transport of ^<131>I-lysozyme across rat intestine in vitro was studied using rat everted jejunum. The transport of lysozyme from the mucosal to serosal side was confirmed by several methods such as TCA-precipitation, immunoprecipitation and enzymatic activity assay. The intestinal absorption of ^<131>I-lysozyme in vivo was studied in rats paying attention to the lymphatics and the portal vein as the route of absorption. The absorption rate of immunoprecipitable ^<131>I via lymphatics was 0.07% of the dose during the first 6 hr after intraintestinal administration of 2 mg/kg of ^<131>I-lysozyme. The absorption rate of immunoprecipitable ^<131>I via portal vein was calculated as 1.99% of ^<131>I-lysozyme dosed, by pharmacokinetic analyses on the concentration of immunoprecipitable ^<131>I in serum. Absorption via lymphatics was 3.24% of the total immunoprecipitable ^<131>I absorbed via both routes. The main route in intestinal absorption of ^<131>I-lysozyme following intraintestinal administration was suggested to be the portal vein, not lymphatics.
公益社団法人日本薬学会の論文
1975-06-25