A Simple Purification Procedure for Rat Pancreatic Elastase and Radioimmunoassay of the Enzyme
スポンサーリンク
概要
- 論文の詳細を見る
Rat pancreatic elastase was purified on a column of lima bean trypsin inhibitor-Sepharose after inactivating trypsin and chymotrypsin in the pancreatic homogenate by treatment with N^α-p-tosyl-L-lysylchloromethyl ketone and N-tosyl-L-phenylalanylchloromethyl ketone. The molecular weight of the enzyme was 25000,as determined by polyacrylamide gel electrophoresis. A radioimmunoassay method was established for rat elastase ; the enzyme level could be determined over the range of 2.5-300 ng of the enzyme per ml.
- 公益社団法人日本薬学会の論文
- 1980-11-25
著者
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片山 幸一
Tsukuba Research Laboratories, Eisai Co., Ltd.,
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大山 俊郎
Department Of Internal Medicine Tokyo Metropolitan Geriatric Hospital
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片山 幸一
Department of Drug Metabolism, Section of Experimental Therapeutics Research, Research & Development
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片山 幸一
Tsukuba Research Laboratories Eisai Co. Ltd.
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片山 幸一
Department of Drug Metabolism and Pharmacokinetics, Tsukuba Research Laboratories, Eisai Company Ltd.
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