Proteome Analysis of Focal Adhesion Kinase (FAK)-Overexpressing Cells(Biochemistry/Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
We established several focal adhesion kinase (FAK) cDNA-transfected cells and found that FAK-transfected HL-60 (HL-60/FAK) cells are resistant to apoptosis induced with hydrogen peroxide, etoposide and radiation compared with the parental HL-60 or the vector-transfected (HL-60/Vect) cells. We carried out proteome analysis to study the mechanism of resistance to apoptosis in HL-60/FAK cells. Among 300 spots resolved in two-dimensional gels, ca. 10% of them were significantly increased in HL-60/FAK cells compared with HL-60/Vect cells, whereas ca. 2% of them were decreased or disappeared. These proteins were performed for further analysis by Western blots or N-terminal sequencing or mass spectrometry. Increased proteins included stress proteins such as hsp90, ribosomal proteins, and antioxidant enzymes such as peroxyredoxin 2. Some of these proteins are assumed to contribute to the antiapoptotic action of FAK.
- 公益社団法人日本薬学会の論文
- 2004-11-01
著者
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Takahashi Reiko
Department of Internal Medicine III, Hamamatsu University School of Medicine
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Kobayashi Takayuki
Cancer Inst. Hospital Tokyo Jpn
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IIJIMA Shiro
Division of Advanced Science and Technology, Kyoritsu College of Pharmacy
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AIZU-YOKOTA Eriko
Department of Biochemistry, Kyoritsu University of Pharmacy
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SONODA Yoshiko
Department of Biochemistry, Kyoritsu University of Pharmacy
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KASAHARA Tadashi
Department of Biochemistry, Kyoritsu University of Pharmacy
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Aizu‐yokota E
Department Of Biochemistry Kyoritsu University Of Pharmacy
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Iijima S
Division Of Advanced Science And Technology Kyoritsu University Of Pharmacy
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Iijima Shiro
Division Of Advanced Science And Technology Kyoritsu College Of Pharmacy
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Sonoda Y
Department Of Biochemistry Kyoritsu University Of Pharmacy
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Sonoda Yoshiko
Department Of Biochemistry And Immunology Kyoritsu University Of Pharmacy
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Takahashi R
Department Of Biochemistry Kyoritsu University Of Pharmacy
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Takahashi Reiko
Department Of Anesthesia Ishikawa Prefectural Central Hospital
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UTSUBO Ryoko
Department of Biochemistry, Kyoritsu University of Pharmacy
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Utsubo Ryoko
Department Of Biochemistry Kyoritsu University Of Pharmacy
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Kasahara T
Department Of Biochemistry Kyoritsu University Of Pharmacy
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Kasahara Tadashi
Department Of Biochemistry And Immunology Kyoritsu University Of Pharmacy
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Takahashi Kaoru
Department Of Breast Oncology Cancer Institute Hospital Of Japanese Foundation For Cancer Research
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Takagi Koichi
Department Of Medical Oncology Cancer Institute Hospital Of Japanese Foundation For Cancer Research
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