Immunogenic Comparison of Two Coupling Methods of Marine Polysaccharide to Bovine Serum Albumin(Pharmacology)
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概要
- 論文の詳細を見る
Two conjugates of marine polysaccharide (MPS) and bovine serum albumin (BSA) were prepared using two methods, periodate oxidation and reductive amination, with the intent of enhancing its immunogenicity. Sera samples from Balb/c mice immunized with the products named MPS-BSAp and MPS-BSAr respectively were evaluated by enzyme-linked-immunosorbent assay (ELISA). The results showed that mice immunized with MPS-BSAp produced antibodies not against MPS but rather against MPS-BSAp, while the mice immunized with MPS-BSAr produced high titer antibodies only specific for MPS. The difference was attributed to the fact that the epitopes of MPS had been changed in the coupling process by periodate oxidation. A mouse immunized with MPS-BSAr was chosen to prepare monoclonal antibodies (mAbs) specific for polysaccharide MPS. A hybridoma cell line that secreted monoclonal antibody recognizing specifically polysaccharide MPS was established.
- 社団法人日本薬学会の論文
- 2004-10-01
著者
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GENG Meiyu
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
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XIN Xianliang
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
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DING Jian
Shanghai Institute of Materia Medica, Chinese Academy of Sciences
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DING Jian
Division of Antitumor Pharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Mat
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Xin Xianliang
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China
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Xin Xianliang
中華人民共和国
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Ding J
Shanghai Institute Of Materia Medica Chinese Academy Of Sciences
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Ding Jian
Institute Of Materia Medica Shanghai Institute For Biological Science Chinese Academy Of Sciences
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Ding Jian
Division Of Anti-tumor Pharmacology State Key Laboratory Of Drug Research Shanghai Institute Of Mate
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Geng Meiyu
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China
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GAN Li
Department of Pharmacology, Marine Drug & Food Institute, Ocean University of China
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Gan Li
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China
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Geng Meiyu
Department of Marine Pharmacology, Marine Drug and Food Institute, Ocean University of China
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