Determination of the Accessibility of Acidic Oligosaccharide Sugar Chain to Blood-Brain Barrier Using Surface Plasmon Resonance(Pharmacology)
スポンサーリンク
概要
- 論文の詳細を見る
There exist limitations in the detection of exogenous oligosaccharides due to their polydisperse and diversiform nature, and particularly the interference of endogenous glycosaminoglycans (GAGs). Herein, a surface plasmon resonance (SPR) assay for detecting acidic oligosaccharide sugar chain (AOSC), an anti-Alzheimer's drug candidate, in cerebrospinal fluid (CSF) was developed based on a carbohydrate antigen-antibody interaction. Rabbits were treated with AOSC intravenously and orally at 40 or 200mg・kg^<-1>, respectively. CSF samples were collected at given time points for quantitative determination of AOSC concentrations in the CSF using an SPR-based competitive inhibition assay, and the existence of AOSC in the CSF was indicated as a blood-brain barrier (BBB) accessibility index. AOSC concentration as low as 50ppb (0.05μg・ml^<-1>) was detected in the CSF, with its peak value approaching 2.091 and 3.316μg・ml^<-1> following intravenous and oral administration, respectively. This is the first time the capacity of AOSC to pass through the BBB has been confirmed using SPR-based competitive inhibition immunoassay. Importantly, the accessibility of AOSC to the BBB indicates AOSC has potential therapeutic value for treating neurodegenerative diseases, particular Alzheimer's disease.
- 社団法人日本薬学会の論文
- 2006-01-01
著者
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GENG Meiyu
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
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XIN Xianliang
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
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Xin Xianliang
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China
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Xin Xianliang
中華人民共和国
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Geng Meiyu
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China
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GUO XiuLi
Department of Pharmacology, Marine Drug & Food Institute, Ocean University of China
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GAN Li
Department of Pharmacology, Marine Drug & Food Institute, Ocean University of China
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NIE Qin
Department of Pharmacology, Marine Drug & Food Institute, Ocean University of China
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Gan Li
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China
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Nie Qin
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China
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Guo Xiuli
Department Of Pharmacology Marine Drug & Food Institute Ocean University Of China:department Of
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Geng Meiyu
Department of Marine Pharmacology, Marine Drug and Food Institute, Ocean University of China
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