Interaction of Protein-Bound Polysaccharide(PSK) with Smooth Muscle Myosin Regulatory Light Chain

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概要

• 論文の詳細を見る

• The interaction of a protein-bound polysaccharide (PSK) isolated from Basidiomycetes with smooth muscle myosin components was evaluated by limited digestion, urea/glycerol gel electrophoresis, affinity chromatography and overlay assay using a peptide array. PSK was bound to the regulatory light chain (RLC) of myosin, but not to the essential light chain. The binding to PSK was definitely observed for unphosphorylated RLC, compared to phosphorylated one. From the amino acid sequence of the RLC, 490 peptides were synthesized on a cellulose membrane. Overlay assays showed that the PSK-binding on the molecule of RLC were localized in the N- and C- terminal basic regions and these sites were conserved in RLC from the human smooth muscle and nonmuscle cells.

• 公益社団法人日本薬学会の論文
• 2003-06-01

著者

• FUJII Toshihiro

  Department of Kansei Engineering, Faculty of Textile Science and Technology, Shinshuu University

• Kunimatsu Mitoshi

  Department Of Biochemistry Ii Nagoya City University Medical School

• Kunimatsu M

  Department Of Biochemistry Nagoya City University Medical School

• Fujii Toshihiro

  Department Of Biological Science School Of Science Nagoya University Nagoya

• Fujii Toshihiro

  Department Of Kansei Engineering Faculty Of Textile Science And Technology Shiushit University Ueda

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