Both HDL_3 and HDL_2 Exert a Powerful Anti-Oxidative and Protective Effect against Acceleration of Oxidative Modification of LDL by Ascorbic Acid

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The objective of the present study was to establish whether high-density lipoprotein 3 (HDL_3) or high-density lipoprotein 2 (HDL_2) might show an anti-oxidative effect on the acceleration of the oxidative modification of low-density lipoprotein (LDL) by ascorbic acid from measurement of the agarose gel electrophoretic mobility of LDL. LDL was incubated without adding transitional-metal ions for 48 or 96h in phosphate-buffered saline (PBS) alone, with ascorbic acid (20μg/mL), or with both ascorbic acid (20μg/mL) and HDL_3 (200μg protein/mL). The LDL autoxidation occurred in PBS alone. Although ascorbic acid significantly suppressed oxidative modification of LDL after incubation for 48h, the opposite was true after 96h. However, since the anti-oxidative ability of HDL_2 shows a weaker tendency than that of HDL_3, both HDL_3 and HDL_2 significantly inhibited this acceleration of oxidative modification of LDL by ascorbic acid as assessed by electrophoretic mobility. If there is an augmented oxidative modification of LDL due to ascorbic acid in vivo, HDL_3 or HDL_2 may thus have an important role in inhibiting this ascorbic acid-accelerated oxidation of LDL.
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