Stimulation of 14-3-3 Protein and Its Isoform on Histamine Secretion from Permeabilized Rat Peritoneal Mast Cells

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概要

• 論文の詳細を見る

• The effect of the 14-3-3 protein, an adaptor protein of intracellular signal pathways, on histamine release from rat peritoneal mast cells was investigated. The exogenous 14-3-3 protein from bovine brain increased the Ca2+-dependent histamine release from permeabilized mast cells, but only slightly affected the non-permeabilized cells. Partial amino acid sequences showed that the bovine brain 14-3-3 protein contained 14-3-3β, γ and ζ isoforms, and that these recombinant isoforms were prepared. Among them, 14-3-3ζ was an active species while the 14-3-3β and γ were inactive for histamine release from the permeabilized mast cells. Approximately 15% of the histamine release was stimulated by 14-3-3ζ at 2.5 μM, and half-maximal stimulation occurred at 1 μM. Treatment of the mast cells with wortmannin or staurosporine completely inhibited the stimulatory effect on histamine release caused by Ca2+ or Ca2+/14-3-3ζ, and genistein partially inhibited both stimulatory effects. PD 98059, however, had little effect on the histamine release. These results suggest the possibility that 14-3-3ζ is associated with signal transduction for degranulation of the mast cells.

• 公益社団法人　日本薬学会の論文
• 2002-12-01

著者

• Fujii Toshihiro

  Department Of Biological Science School Of Science Nagoya University Nagoya

• UEEDA Takayuki

  Department of Kansei Engineering, Faculty of Textile Science and Technology, Shinshu University

• Fujii T

  Department Of Kansei Engineering Faculty Of Textile Science And Technology Shinshu University

• Ueeda Takayuki

  Department Of Kansei Engineering Faculty Of Textile Science And Technology Shinshu University

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