Increase of Intracellular Glutathione by Low-Dose γ-Ray Irradiation Is Mediated by Transcription Factor AP-1 in RAW 264.7 Cells(Biochemistry/Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
The mechanism of the elevation of intracellular glutathione induced by low-dose γ-rays was examined in RAW 264.7 cells. The expression of mRNA for γ-glutamylcysteine synthetase (γ-GCS) increased soon after γ-ray (0.5 Gy) irradiation, and peaked between 3 h and 6 h post-irradiation. A dose of 0.25 to 0.5 Gy was optimum for induction of γ-GCS mRNA expression at 3 h post-irradiation. The effect of inhibitors of activator protein-1 (AP-1) and nuclear factor κB (NF-κB) on the radiation-induced γ-GCS gene expression was then examined. The induction of γ-GCS mRNA expression was significantly suppressed when AP-1 DNA binding, but not NF-κB DNA binding, was inhibited. Finally, electrophoretic mobility shift assay showed that the low-dose radiation markedly increased the DNA binding of AP-1, but not NF-κB, soon after irradiation. These results suggest that the increase of glutathione levels in RAW 264.7 cells by low-dose γ-ray irradiation is mediated by transcriptional regulation of the γ-GCS gene, predominantly through the AP-1 binding site in its promoter.
- 公益社団法人日本薬学会の論文
- 2003-01-01
著者
-
Kojima Shuji
Faculty Of Pharmaceutical Sciences Science University Of Tokyo
-
Ikekita Masahiko
Faculty Of Science And Technology Tokyo University Of Science
-
Kojima Shuji
Faculty Of Pharmaceutical Sciences Tokyo University Of Science
-
Kurozumi Risa
Faculty Of Pharmaceutical Sciences Tokyo University Of Science
-
KAWAKITA Yasunori
Faculty of Pharmaceutical Sciences, Tokyo University of Science
-
Kawakita Yasunori
Faculty Of Pharmaceutical Sciences Tokyo University Of Science:faculty Of Science And Technology Tok
-
Kurozumi R
Faculty Of Pharmaceutical Sciences Tokyo University Of Science
関連論文
- Effect of Heterocyclic Pyrimidine Compounds on UVB-Induced Cell Damage in Human Keratinocytes and on Melanogenesis in Mouse B16 Cells
- Low-Dose Gamma-Ray Irradiation Induces Translocation of Nrf2 Into Nuclear in Mouse Macrophage RAW264.7 Cells
- Immune Response Pathways in Human Keratinocyte (HaCaT) Cells are Induced by Ultraviolet B via p38 Mitogen-activated Protein Kinase Activation
- A comparative study of nitric oxide, glutathione, and glutathione peroxidase activities in cerebrospinal fluid from children with convulsive diseases/children with aseptic meningitis
- CD69-null mice protected from arthritis induced with anti-type II collagen antibodies
- 糖鎖導入ドラッグデリバリー材料開発のためのネオ糖タンパク質・リポソームコンジュゲートの調製と機能評価
- Inhibitory Effects of Prior Low-dose X-ray Irradiation on Carbon Tetrachloride-induced Hepatopathy in Acatalasemic Mice
- Basic Study on the Internal Movement of Inhaled Radon -for Establishment of Experimental Methods -
- Prolonged skin allograft survival by IL-10 gene-introduced CD4 T cell administration
- The Elevation of p53 Protein Level and SOD Activity in the Resident Blood of the Misasa Radon Hot Spring District
- 22 Biochemical Comparison between Radon Effects and the Other Effects on Humans in Radon Hot Spring Therapy(Low dose and low dose-rate effects, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Elevation of Antioxidant Enzymes in the Clinical Effects of Radon and Thermal Therapy for Bronchial Asthma
- Effects of Radon Therapy on Bronchial Asthma
- Mechanisms of Alleviation of Degeneration or Deforming Diseases of Joints by Radon Therapy
- Radon Effects and Thermal Effects on Humans in Radon Therapy
- Immunological effects of The early Radon Therapy at the Misasa Hot Spring
- The Chemical Biological Defense Mechanisms on the Early Radon Therapy at the Misasa Hot Spring
- Suppression of Diabetes by Low Dose γ-Ray Irradiation in Nonobese (NOD) Mice
- Effects of Low Dose Irradiation on Mouse Brain Oxidized by Fe^-NTA
- Inhibitory Effects of Low Dose Irradiation on Fe^ -NTA-induced Hepatopathy in Mice
- Effects of Post Low-dose γ-ray Irradiation on Fatty Liver Induced by Carbon Tetrachloride
- Induction of Glutathione in Mouse Brain by Low Doses of γ-Rays and Its Applicability to Protection and Therapy against Brain Diseases
- Effect of Small Doses of γ-Ray on the Glutathione Level in Mouse Organs.
- Does Small-Dose γ-Ray Radiation Induce Endogenous Antioxidant Potential in Vivo?
- Suppressing Effect of Low-Dose Gamma-Ray Irradiation on Collagen-Induced Arthritis
- Characterization of molecules recognized by antibody against nerve growth cone in the rat brain and intestine
- ^ Ga-CITRATE ACCUMULATION IN TUMOR CELLS AND HEPARAN SULFATE
- Effect of Repeated Small-Dose γ-Ray Irradiation on Atopic Dermatitis in NC/Nga Mice
- Repeated Irradiations with γ-rays at a Dose of 0.5 Gy May Exacerbate Asthma
- Low-level Nitric Oxide Blunts Oxidant Injury via Up-regulating Glutathione Synthesis
- Involvement of Mitochondrial Peroxynitrite in Nitric Oxide-Induced Glutathione Synthesis(Biochemistry/Molecular Biology)
- Increase of Intracellular Glutathione by Low-Dose γ-Ray Irradiation Is Mediated by Transcription Factor AP-1 in RAW 264.7 Cells(Biochemistry/Molecular Biology)
- Involvement of P2Y receptors in the protective effect of ATP towards the cell damage in HaCaT cells exposed to H_2O_2
- High resolution SPECT imaging for visualization of intratumoral heterogeneity using a SPECT/CT scanner dedicated for small animal imaging
- Relationship between peroxisome proliferator-activated receptor-γ activation and the ameliorative effects of ascochlorin derivatives on type II diabetes