ニシン卵の受精 : VII. 卵門域の数種の色素に対する親和性

概要

論文の詳細を見る
The micropyle area of the mature egg has a specific affinity to some vital dyes. When fresh unfertilized eggs were treated with a dilute (0.05 per cent) aqueous solution of toluidine blue for 2 or 3 minutes and rinsed in tap water, the surfaces of the egg membrane were stained reddish purple (metachromatic shade) in marked contrast to the pale blue hue of the remaining area of the egg membrane (cf. Figs. 1 and 2). Using a more dilute (0.2 per cent) aqueous solution of brilliant cresyl blue, a similar result was also obtained. It has been also found that the same area is stained more or less intensely with certain other basic dyes, such as methylen blue, azure B, Nile blue S, and thionine. Furthermore, it has been established that the micropyle canal and its vicinity, which have little affinity to the abovelisted dyes, are intensely stained with Janus green B, Janus red, Victoria blue 4 R, and aniline blue. For example, when unfertilized eggs were immersed in a 0.5 per cent aqueous solution of Janus green for 5 to 15 minutes, and washed in acidified water (pH 3-4) for a few minutes, the micropyle canal and the surfaces of the egg membrane in the vicinity of the micropyle opening were stained deep blue (cf. Fig. 3). As has been reported previously (Yanagimachi, '57 c, d), the surface of the egg membrane of the micropyle area contains some specific sperm-activating factor, the presence of which is requisite for normal fertilization. This factor is easily destroyed by treatment with some agents, such as acidified (pH 3.5) Ringer's solution, trypsin Ringer's solution, and isotonic (M/6.7) CaCl_2 solution and by the incubation at 60〜70℃ in Ringer's solution. Some experiments have shown that the substances responsible for the specific affinity to toluidine blue and Janus green are fairly resistant to the agents described above. Therefore, it may be reasonable to assume that some substances responsible for the affinity to toluidine blue, Janus green, and certain other dyes are not the sperm-activating factor itself. Tentatively, the author has supposed that the substance in question might be a carrier to which the sperm-activating factor is closely bound.
社団法人日本動物学会の論文
1958-10-15

ニシン卵の受精 : VII. 卵門域の数種の色素に対する親和性

スポンサーリンク

概要

著者

関連論文

スポンサーリンク