フナの黒色素胞およびその調節神経の機能に対する放射線の影響

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概要

• 論文の詳細を見る

• In the present paper, the effect of γ-irradiation on the physiological responses of nerve-melanophore system in the fish, Carassius carassius, is reported. Isolated split preparations of the caudal fin of Carassius were used as test materials. A solution of adrenalin (10^<-5>M) which is known to act directly on the melanophore itself, was applied as a concentrating agent, and an atropine solution (5×10^<-5>M), was a disperser. In order to stimulate the nerve endings KCl (M/7.5) was used as a concentrating agent, and NaCl (M/7.5), as a dispersing agent. Stimulation by electric current (1V/mm of AC) was used for excitation of the concentrating nerve fibers. Responses of melanophores to these stimulations were recorded with the photoelectric recording method described by Fujii (1959). The results are shown in Tables 1, 2 and 3, and Fig. 3. At first, the responses of the melanophores to adrenaline or atropine were recorded for each preparation, and then they were irradiated with ^<60>Co γ-rays at the doses of 62.5, 125, 250, 375, 500 and 625kr (dose rate was about 12.5kr/min in air). After irradiation, the preparation were retested with the same chemical drugs. Dose-response curves of irradiated malanophores were sigmoid Weak but definite responses to both agents were still recognizable, even after 625kr or γ-irradiation (Fig. 3). In addition to adrenaline and atropine, salt solutions of KCl or NaCl were used for stimulation of the nerve endings. Measurements were conducted in the same manner as for of adrenaline and atropine. The doses used were, however, 62.5, 125, 250 and 375kr. The results are also shown in Fig. 3, and the curves were convex shape in this case. Both the responses to KCl and NaCl disappeared after exposure to 375kr or γ-rays. To clarify the effect of irradiation on the concentrating nerve fibers of the melanophores, electrical stimulation was used. Before and after γ-irradiation with the doses of 62.5, 125kr, the responses to the electric curret were recorded. The result similar to that for was obtained as to that for concentrating nerve endings was obtained. But, in the nerve fibers, the response to electrical stimulation throughly disappeared after irradiation at 250kr of γ-rays (Fig. 3). The data so far obtained suggest that radiation has a more harmful effect on the dispersing processes of the nerve-melanophore system than on the concentrating processes. A comparison of the various responses of the nerve-melanophore system to above treatments seem justify the radiosensitivity of the nerve fibers is the highest, that of the meanophore is the lowest, and that of the nerve endings lies between the two.

• 社団法人日本動物学会の論文
• 1963-10-15

著者

• 江藤 久美

  放射線医学総合研究所生物研究部

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• フナの黒色素胞およびその調節神経の機能に対する放射線の影響

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