3倍体ギンブナのgynogenesisに関する細胞学的研究

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The cytological process from insemination to second cleavage was investigated in the cross between the female ginbuna (C. auratus langsdorfi) from Kanto district and the male kinbuna (C. auratus subsp.). The former had the triploid chromosome number of 156, and the latter had the diploid chromosome number of 100. Eggs of the ginbuna were artificially inseminated with kinbuna sperm at water temperature from 20℃ to 22℃. About 2 minutes after insemination, a single spermatozoon of the kinbuna entered the ginbuna egg, and the second maturation division of the egg proceeded. The extrusion of the second polocyte followed, and then the sperm aster developed. In spite of the fact that the egg nucleus was transformed into the female pronucleus about 22 minutes after insemination, the sperm nucleus remained as it was without any swelling. About 30 minutes after insemination, the sperm nucleus was found to contact with the female pronucleus. However, no amphimixis was observed, and the first karyokinesis was found only in the female pronucleus. The eggs proceeded to metaphase or anaphase stage of the first karyokinesis about 42 minutes after insemination. During the first karyokinesis the sperm nucleus remained as a condensed mass in contact with a part of the spindle. Two blastomeres, in which the daughter nuclei reconstructed, were formed about 52 minutes after insemination. At this time, the sperm nucleus still remained in the cytoplasm of one of the two blastomeres. The second polar body was found in its original position at the first cleavage. It was shown clearly that in this cross gynogenesis took place in the eggs of the triploid ginbuna.
社団法人日本動物学会の論文
1971-09-15

3倍体ギンブナのgynogenesisに関する細胞学的研究

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