小型種族の大型種族のシマドジョウの染色体について

概要

論文の詳細を見る
Subjected to the present chromosome study were four smallrace spinous loaches (2♀ and 2♂, TL. 6.3-9.5cm) collected in Tokyo and Saitama Prefecture and three largerace spinous loaches (2♀ and 1♂, TL. 8.3-12.8cm) collected in Wakayama and Gifu Prefectures. Cells obtained from the gonadal tissues were cultured for 7 days in medium TC-199 supplemented with 20% calf serum, 100 U./ml penicillin and 100μg/ml streptomycin. Chromosome preparations were made according to the standard flame-drying method. The small race of spinous loaches had a diploid chromosome number of 48, consisting of 9 pairs of metacentrics and 15 pairs of submetacentric elements. On the other hand, the large race of spinous loaches showed 96 chromosomes in diploid number. The karyotype consisted of 18 pairs of metacentrics, 26 pairs of submetacentrics and 4 pairs of subtelocentric elements. There was no morphological difference between male and female karyotypes in either race. Based on the comparison of karyotypes in the two races of spinous loaches, it is suggested that the small race represents a diploid state, while the large race exists in a tetraploid state, although it is not an exact duplication of chromosomes.
社団法人日本動物学会の論文
1976-03-25