非イオン界面活性剤によるプロテアーゼの活性上昇の速度論的研究(第2報)

概要

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Our previous study discussed kinetically the enhancement of alkali protease activity toward casein by the presence of nonionic surfactants, C_<12>(EO)_7, C_<16>(EO)_<15> and C_<8φ>(EO)_<10>. In the present work, study has been extended to the systems containing C_<16>(EO)_7, C_<9φ>(EO)_<10> and C_<9φ> (EO)_<15> ranging in HLB values from 11.0 to 15.0. It was confirmed again that the activity enhancement is observed for the surfactant systems and that the experimental results are explained by assuming in the kinetic scheme proposed previously that the dissociation constant for enzyme-micelle-substrate is reduced to one several tenth of that for enzyme-substrate. On the contrary, it was found for the surfactants to reduce the enzyme activity toward a model substrate, glutaryl-Gly-Gly-Phe-βNa. This suggests that the aforementioned enhancement is specific to macromolecular substrate.
社団法人日本家政学会の論文
1991-04-15