HISTOCHEMICAL STAINING OF CELLS CONTAINING FLAVOENZYME _D-AMINO ACID OXIDASE BASED ON ITS ENZYMATIC ACTIVITY : APPLICATION OF A COUPLED PEROXIDATION METHOD
スポンサーリンク
概要
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A histochemical method for the demonstration of D-amino acid oxidase activity in rat kidney, liver and brain is described. The method is a peroxidase-coupled procedure, which is based on the intensifying effect of nickel ions on 3,3'-diaminobenzidine-based product formation with peroxidase. The substrates used were D-proline and D-alanine, of which the former showed more intense staining. The histochemical reaction was suppressed by benzoate, a competitive inhibitor. There was no staining on omission of a substrate or peroxidase from the incubation medium. The method was specific for D-amino acid oxidase. The oxidase in kidney and brain showed resistance to the fixatives used (e.g. 8% glutaraldehyde), while the enzyme in hepatocytes did not. The reaction product was black, electron dense, stable and strictly confined to cells containing the oxidase. The method was successfully applied to the histochemical identification of the type of cell containing the oxidase at both the levels of light and electron microscopy. The oxidase activity was shown to be present in hepatocytes in the liver, proximal tubule cells in the kidney, and only astrocytes, including Bergmann glial cells, in the brain. In the midbrain, the oxidase was almost. exclusively restricted to the tegmentum.
- 日本組織細胞化学会の論文
著者
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Maeda Toshihiro
Department of Cardiology, Komatsushima Red Cross Hospital
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TOJO Hiromasa
Department of Biochemistry and Molecular Biology, Osaka University Graduate School of Medicine
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Arai Ryohachi
Department of Anatomy, Shiga University of Medical Science
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YAMANO Toshio
Department of Biochemistry, Osaka University Medical School
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Arai Ryohachi
Department Of 1st Anatomy Shiga University Of Medical Science
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Arai R
Department Of Anatomy
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Arai Ryohachi
Departments Of Anatomy Shiga University Of Medical Science
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Nozaki M
Osaka Univ. Osaka
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HORIIKE Kihachiro
Department of Biochemistry, Shiga University of Medical Science
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NOZAKI Mitsuhiro
Department of Biochemistry, Shiga University of Medical Science
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Maeda Toshihiro
Department Of Anatomy Shiga University Of Medical Science
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Maeda Toshihiro
Department Of Anatomy Of Shiga University Of Medical Science
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Tojo H
Osaka Univ. Medical School Osaka Jpn
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Tojo Hiromasa
Department Of Biochemistry And Molecular Biology Graduate School Of Medicine Osaka University
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Yamano T.
大阪市立大学 小児科
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Nozaki M
Departments Of Biochemistry Shiga University Of Medical Science
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Yamano Toshio
Department Of Biochemistry Medical School Osaka University
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Horiike Kihachiro
Dept Biochem Mol Biochem, Shiga Univ Med Sci
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Horiike K
Dept Biochem Mol Biochem Shiga Univ Med Sci
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Horiike Kihachiro
Department Of Biochemistiy Shiga University Of Medical Science
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