HISTONE H1 DEPLETION WITH HUMAN CELLULAR AGING : BIOCHEMICAL AND CYTOCHEMICAL EVIDENCES (<Symposium>Histo- and Cytochemistry in Tissue Culture)
スポンサーリンク
概要
- 論文の詳細を見る
Biochemical and immunocytochemical examinations of histone H1 in G1 arrested and senescent human fibroblasts were performed to pursuit the mechanisms of human cell aging in vitro. Examinations of histone synthesis in serum depleted or contact inhibited cell cultures indicated that the ratio of histone synthesis over DNA synthesis and molar synthesis of histone H1 to nucleosomal histones did not decline with accumulation of resting cell populations, while senescent cells had a lower ratio of histone H1 synthesis than young cells. Histone H1 contents plotted as a function of DNA synthesis activity revealed that senescent cultures had a lower content of histone H1 than young cultures at any stage of cell proliferation. Immunocytological study using antiserum against histone H1 on cocultivated young and senescent cells showed that immunofluorescence intensity was very low in senescent nuclei in comparison with young cell nuclei. Thus, we conclude that a decline in content and synthesis of histone H1 relative to nucleosomal histones with passage number was not simply due to the passage-related accumulation of resting cells, but actually reflects age-specific changes in cycling cells or whole cell populations in the senescent cultures. The depletion of histone H1 in chromatin was discussed as a possible cause of DNA strand break and relaxation of gene repression.
- 日本組織細胞化学会の論文
著者
-
Sakagami Hiroshi
Department of Health and Nutrition Sciences,Tokyo Seiei College
-
Mitsui Youji
Department Of Biochemical Pharmacology Tokyo Metropolitan Institute Of Gerontology
-
Sakagami Hiroshi
Department Of Biochemical Pharmacology Tokyo Metropolitan Institute Of Gerontology
関連論文
- Steroidal Glycosides from Furcraea foetida and Their Cytotoxic Activity
- Chemical Constituents of the Bulbs of Habranthus brachyandrus and Their Cytotoxic Activities
- Triterpene Glycosides from the Whole Plant of Anemone hupehensis var. japonica and Their Cytotoxic Activity
- 再録報文 Radical Scavenging Activity and Inhibition of Macrophage NO Production by Fukinolic Acid,a Main Phenolic Constituent in Japanese Butterbur(Petasites japonicus)
- Biological activity of a fruit vegetable, "Anastasia Green", aspecies of sweet pepper
- Cytotoxic Activity of 2-Aminomethylene-3(2H)-benzofuranones against Human Oral Tumor Cell Lines
- Cytotoxic Activity of Benzothiepins against HumanOral Tumor Cell Lines
- Cytotoxic Activity of 5-Benzoylimidazole and RelatedCompounds against Human Oral Tumor Cell Lines
- α-Trifluoromethylacyloins Induce Apoptosis in HumanTumor Cell Lines
- Convallasaponin A, a New 5β-Spirostanol Triglycoside from the Rhizomes of Convallaria majalis
- Norlanostane and Lanostane Glycosides from the Bulbs of Chionodoxa luciliae and Their Cytotoxic Activity
- Cycloartane Glycosides from the Rhizomes of Cimicifuga racemosa and Their Cytotoxic Activities
- Five New Triterpene Glycosides from Centella asiatica
- Steroidal Glycosides from the Bulbs of Camassia leichtlinii and Their Cytotoxic Activities
- Polyphenols from Eriobotrya japonica and Their Cytotoxicity against Human Oral Tumor Cell Lines
- Triterpene Glycosides from the Tubers of Anemone coronaria
- Azadirachta indica, a tropical medicinal plant with possible utility for the treatment of parasitoses
- A brief note on the Sannaimaruyama-Iseki Remains of the Jomon period in Aomori Prefecture with special comments on paleoparasitological aspects
- Construction of the Curriculum and Lectures for the Subject, "History of Pharmacy" in Matsuyama University School of Pharmacy
- A Chronological Research on Parasitic Endemic Disease, "Katayama-disease" (schistosomiasis japonica) in Hiroshima Prefecture in the 20th Century : The 3rd Communication on the Historical Research on schistosomiasis japonica in Hiroshima Prefecture
- Spontaneous disappearance of a renal arteriovenous malformation
- HISTONE H1 DEPLETION WITH HUMAN CELLULAR AGING : BIOCHEMICAL AND CYTOCHEMICAL EVIDENCES (Histo- and Cytochemistry in Tissue Culture)
- Furostanol glycosides from the rhizomes of Helleborus orientalis
- Intravesical instillation of hydroxypropylcelluloseadriamycin and combined treatment with hyperthermia for bladder cancer
- Five New Triterpene Glycosides from Centella asiatica
- Polymethoxylated Flavonoids from the Peels of Citrus depressa(Natural Medicine Note)
- Polymethoxylated Flavonoids from the Peels of Citrus depressa