CHEMICAL FACTORS IN THE IODINE COLORATION REACTION EMPLOYED IN THE HISTOCHEMICAL DEMONSTRATION OF PHOSPHORYLASE

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The influences of primer glycogen on the iodine staining procedure for histochemical demonstration of phosphorylase were studied by biochemical, physico-chemical and electrophoretical techniques. The low concentration of primer present in the in vitro reaction gave the blue color with iodine staining for polysaccharide synthesized by phosphorylase and the high concentration of primer glycogen exhibited the reddish color. The muscle type glycogen had the stronger affinity to the iodine solution than the liver type. Polygucose which was synthesized by the simultaneous action of phosphorylase and glycosyl transferase was stained reddish with iodine. Each polyglucose which was synthesized by the activity of isoenzymes of the liver, muscle and hepatoma AH13 stained similarly with iodine following electrophoresis, in spite of a different coloration in the histochemical procedure. In the latter, it is likely that polyglucose synthesized in the intracellular conditions of the incubated tissue might be influenced by the activity of enzymes as well as the concentration of primer glycogen and it's physico-chemical structure
日本組織細胞化学会の論文