ELECTRON HISTOCHEMICAL OBSERVATIONS OF POLYSACCHARIDE SYNTHESIZED BY ENZYME ACTIVITIES IN RAT LIVER CELLS
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概要
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Intracellularly synthesized polysaccharide from glucose-1-phosphate by phosphorylase and amylo-1,4⇾1,6-transglucosidase in rat liver cells was observed by histochemical and electron microscopical procedures. This polysaccharide stained red-purple with iodine under light microscope. It was completely digested by α-amylase, while in completely digested by β-amylase. Electronmicroscopically, newly synthesized polysaccharide appeared to be finer granules than β-granules of native glycogen. These new granules were present as amorphous aggregates or monoparticulates, but very often had a tendency to make irregular rosettes, different from native glycogen. They stained less with lead in density on electron microphotograph and were more sensitive to electron beam than native glycogen particulates.
著者
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Takeuchi Tadao
Department Of Pathology Kumamoto University School Of Medicine
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Sasaki Mitsuo
Department Of Environmental Engineering Kitami Institute Of Technology
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