HISTOCHEMICAL AND ELECTRON MICROSCOPIC DIFFERENCES BETWEEN NATIVE GLYCOGEN AND POLYGLUCOSE SYNTHESIZED BY PHOSPHORYLASE IN TISSUE CELLS
スポンサーリンク
概要
- 論文の詳細を見る
Polysaccharide formed histochemically from glucose 1-phosphate by the enzyme activity and native glycogen were observed under the electron microscope. Polyglucose newly formed only by phosphorylase in rat muscle fibers was stained blue with iodine and appeared to be a finer granular evidence showing ambiguous particles with a diameter from 100Å to 150Å, while native glycogen stained red brown with iodine was demonstrable in monoparticulate form with diameter from 200Å to 400Å. Polysaccharides synthesized by phosphorylase and branching enzyme in rat liver and hepatoma cells were stained red purple with iodine and were demonstrable in the same ambiguous granular form with a diameter from 100Å to 200Å, while native glycogen appeared to form so-called glycogen rosettes. In the methacrylate-embedded sections, the newly synthesized polyglucose was more sensitive to the electron beam and thereby was more easily degraded and missed in the cells than native glycogen. When using the Epon embedding the resistance of both substances to the electron beam increased markedly. Nevertheless, the newly formed polyglucose was less resistant to the beam than native glycogen. Furthermore, the synthesized polyglucose had difficult staining with lead hydroxide, while glycogen stained densely with it. It was, therefore, newly found that polyglucose synthesized from glucose 1-phosphate by the enzyme activity is obviously different from native glycogen, contrary to Leloir's in-vitro observation.
著者
-
Takeuchi Tadao
Department Of Pathology Kumamoto University School Of Medicine
-
Sasaki Mitsuo
Department Of Pathology Kumamoto University School Of Medicine Kumamoto
-
Sasaki Mitsuo
Department Of Environmental Engineering Kitami Institute Of Technology
-
Takeuchi Tadao
Department Of Pathology Kumamoto University School Of Medicine Kumamoto
関連論文
- Studies on the Mechanism of Glycogenosome Formation (II)Biochemical Properties and Immunohistochemistry of Neutral α-Glucosidase
- Study on the Mechanism of Glycogenosome Formation (8) : Molecular Diversity of α-Glucosidases
- Study on the Mechanism of the Glycogenosome Formation (7) : Heterogeneity of Glycogen Macromolecules and Autophagic Activity
- Effect of 3mg melatonin on jet lag syndrome in an 8-h eastward flight
- Sleep architectures of obstructive sleep apnea syndrome in the young child
- Re-entrainment of circadian rhythm of plasma melatonin on an 8-h eastward flight
- Clinical features of circadian rhythm sleep disorders in outpatients
- New CR System with Pixel Size of 50μm for Digital Mammography : Physical Imaging Properties and Detection of Subtle Microcalcifications
- HIGH PERFORMANCE REVERSED-PHASE LIQUID CHROMATOGRAPHY OF TRACE AMOUNTS OF INORGANIC AND ORGANIC MERCURY AS DIETHYLDITHIOCARBAMATE CHELATES
- A Cytochemical Study of the Intracellular Localization of Uridine Diphosphate Glucose Glycogen Glucosyltransferase, Using the Centrifugal Fraction Techniques and Electron Microscope
- A Cytochemical Study of the Intracellular Localization of Phosphorylase, using the Centrifugal Fraction Technique and Electron Microscope
- ULTRASTRUCTURE AND PHYSICO-CHEMICAL PROPERTIES OF GLYCOGEN FROM FETAL RAT LIVER
- The Application of the Laser Microprobe Spectral Analyser for the Quantitative Enzyme Histochemistry
- Intracellullar Synthesis of Macromolecular Polyglucose Particles in Mammalian Tissue Cells and its Biological Significance
- MACROMOLECULAR GLYCOGEN SYNTHESIS INDUCED FROM ENZYME HISTOCHEMISTRY
- Localization and structure of glycogen particles histochemically synthesized by enzymes
- Electron Histochemical Observations of Enzyme-synthesized Polyglucoses Stained Differently with Iodine, Using Rat Skeletal Muscle Fibers
- Electron-histochemical Observation of UDPglucose-glycogen Glucosyltransferase Reaction
- ELECTRON HISTOCHEMICAL OBSERVATIONS OF POLYSACCHARIDE SYNTHESIZED BY ENZYME ACTIVITIES IN RAT LIVER CELLS
- HISTOCHEMICAL AND ELECTRON MICROSCOPIC DIFFERENCES BETWEEN NATIVE GLYCOGEN AND POLYGLUCOSE SYNTHESIZED BY PHOSPHORYLASE IN TISSUE CELLS
- Picornavirus-like Inclusion in Poly-myositis is an Aggregation of Glycogen Particles in the Same Size
- Polysaccharide Staining of Newly Formed Glycogen Particles by the Glycogen Synthetase Activity
- Histochemical and Electron Microscopic Observations of New Polyglucoses Synthesized from UDPG, TDPG and GDPG by the Glycogen Synthetase Activity in the Rat Tissues.
- Ultra-and Physico-chemical Structure of the Fetal Glycogen Particle and Some Related Enzymes in its Metabolism
- Light and Electron Microscopic Properties of Polyglucose Intracellularly Synthesized by Enzymes
- Electrophoretical Study of Phosphorylase Isozymes
- CHEMICAL FACTORS IN THE IODINE COLORATION REACTION EMPLOYED IN THE HISTOCHEMICAL DEMONSTRATION OF PHOSPHORYLASE
- Reinvestigation on the Stainability of Polysaccharide Synthesized from Glucose-1-Phosphate by the Enzyme Activity with Iodine Reaction
- Electron-histochemical Observations of Synthesized Polyglucose Particles in the Blood Capillary
- INTRANUCLEAR SYNTHESIS OF POLYGLUCOSE PARTICLES UNDER HISTOCHEMICAL CONDITIONS IN THE NORMAL RAT
- Histochemical Study of Peripheral Nerve Lesions Induced by Methyl Mercury Chloride
- Alkaline Phosphatase of Cancer Cells in vivo and in vitro
- AN ELECTRON HISTOCHEMICAL OBSERVATION OF POLYSACCHARIDE SYNTHESIZED FROM URIDINE DIPHOSPHOGLUCOSE BY THE ENZYME ACTIVITY
- Electron-Histochemical Observation of Polysaccharide Produced by Phosphorylase Reaction in Rat Liver Parenchymal Cells
- Electron-histochemistry of a Polyglucose Synthesis by Phosphorylase in Rabbit Small Intestine Smooth Muscle
- Histochemical Demonstration of Mercury by Use of Autoradiographic Procedure Report II; Distribution of Mercury in Human Autopsy Cases of Minamata Disease
- HISTOCHEMICAL DEMONSTRATION OF MERCURY IN HUMAN TISSUE CELLS OF MINAMATA DISEASE BY USE OF AUTORADIOGRAPHIC PROCEDURE
- LOCALIZATION OF ALKALINE PHOSPHATASE ISOZYMES IN HUMAN CANCER CELL LINES IN VITRO
- ELECTRON-HISTOCHEMICAL DEMONSTRATION OF PHOSPHORYLASE AND ITS INTRACELLULAR DISTRIBUTION IN RAT SKELETAL MUSCLES
- On the Specificity of Histochemical Demonstration for Glucosephosphate Isomerase
- HISTOCHEMICAL STUDY OF ENZYMES, PARTICULARLY IN CARBOHYDRATE METABOLISM OF THE BOVINE HEART CONDUCTION SYSTEM
- Histochemical Aspect of Normal Liver Cells, Particularly as the Fundamental Knowledge for Organ Transplantation (Antigenicity and Transplantation of the Liver)