IMMUNOHISTOCHEMICAL STUDY OF γ-GLUTAMYL TRANSPEPTIDASE DURING THE DEVELOPMENT AND MATURATION OF RAT KIDNEY
スポンサーリンク
概要
- 論文の詳細を見る
Gamma-glutamyl transpeptidase (γ-GTP) was localized in prenatal and neonatal rat kidneys using an immunohistochemical method with monoclonal antibodies to rat kidney γ-GTP. γ-GTP was demonstrated on the brush border and basolateral membrane of proximal convoluted tubules from the 18th fetal day. The basolateral membrane of proximal tubules revealed strong reaction at 1 and 3 days after birth. The reaction of basolateral membrane decreased with the maturation of nephrons, however, moderate reaction was still observed at 30 days of age in the S3 segment. Throughout the postnatal development of the kidney, the S3 exhibited the strongest immunostaining and S1 showed the weakest reaction, and proximal tubules in the deep layer of the cortex always provided a stronger reaction than those in the superficial layer of the cortex. Maturating cuboidal and squamous epithelial cells of descending Henle's loop showed a positive reaction on the luminal and lateral cell membranes. On immunoelectron microscopy, γ-GTP was found on the brush border, basolateral membrane, membranes of pinocytotic vesicles and apical invaginations in proximal tubular cells at 1-7 days after birth. However, tight junctions were devoid of the reaction. The development of basal infolding and localization of γ-GTP there seemed to relateclosely to the development of surrounding capillaries. γ-GTP was localized on the cell membrane and occasionally in the cytoplasm of interstitial cells of renal interstitium.
- 日本組織細胞化学会の論文
著者
-
Yasuda K
Laboratory Of Metabolism Graduate School Of Human And Environmental Studies Kyoto University
-
Niu Jian-zhao
Dapartment Of Anatomy School Of Medicine Keio University
-
YAMASHITA SHUJI
Dapartment of Anatomy, School of Medicine, Keio University
-
YASUDA KENJIRO
Dapartment of Anatomy, School of Medicine, Keio University
-
Yamashita Shuji
Dapartment Of Anatomy School Of Medicine Keio University
-
Yasuda Kenjiro
Dapartment Of Anatomy School Of Medicine Keio University
関連論文
- Antihyperglycemic Effect of T-1095 via Inhibition of Renal Na^+ -Glucose Cotransporters in Streptozotocin-Induced Diabetic Rats
- Correction of Hyperglycemia and Insulin Sensitivity by T-1095, an Inhibitor of Renal Na^+-Glucose Cotransporters, in Streptozotocin-Induced Diabetic Rats
- Morphological Changes and lucreased Sucrace and Isomaltase Activity in Small Intestines of Insulin-Deficient and Type 2 Diabetic Rats
- Sucrasc-Isomaltase Gene Expression Is Inhibited by Mutant Hepatocyte Nuclear Factor (HNF)-1α and Mutant HNF-1β in Caco-2 Cells
- Growth-Related Changes in Skeletal Muscle Fiber Type and Insulin Resistance in Diabetic Otsuka Long-Evans Tokushima Fatty Rats
- INTRACELLULAR LOCALIZATION OF ACID PHOSPHATASE AND TRYPSINOGEN ON AN IDENTICAL SPECIMEN IN RAT PANCREAS
- NEW EMBEDDING METHOD FOR IMMUNOHISTOCHEMICAL STUDIES USING ACRYLAMIDE GEL
- Immunohistochemical Study of Secretory Granule Membrane
- MICROWAVE FIXATION: EXAMINATION OF TEMPERATURE IN TISSUES DURING IRRADIATION (Microwave Fixation in Histochemistry)
- MONOCLONAL ANTIBODIES TO THE SECRETORY GRANULE MEMBRANE OF THE RABBIT PAROTID GLAND : PRESENCE OF A COMMON ANTIGEN IN SECRETORY GRANULES OF BOTH EXOCRINE AND ENDOCRINE CELLS
- IMMUNOHISTOCHEMICAL STUDY OF GAMMA-GLUTAMYL TRANSPEPTIDASE WITH MONOCLONAL ANTIBODIES : II. AN IMMUNOELECTRON MICROSCOPIC STUDY IN RAT KIDNEY
- AN EXPERIMENTAL STUDY ON THE APPLICATION OF MICROWAVE FIXATION TO IMMUNOHISTOCHEMICAL STUDIES (Some Recent Advances in Life Sciences : Modern Histo- and Cytochemistry and Their Future)
- AN APPLICATION OF CRYOFIXATION TO IMMUNOCYTOCHEMISTRY
- PREPARATION OF MONOCLONAL ANTIBODY TO RABBIT KIDNEY GAMMA-GLUTAMYL TRANSPEPTIDASE AND ITS CROSS-REACTIVITY ANALYSIS IN IMMUNOHISTOCHEMISTRY
- IMMUNOHISTOCHEMICAL STUDY OF γ-GLUTAMYL TRANSPEPTIDASE WITH MONOCLONAL ANTIBODIES : I. PREPARATION AND CHARACTERISTICS OF MONOCLONAL ANTIBODIES TO γ-GLUTAMYL TRANSPEPTIDASE
- Immunohistochemical study of secretory granule membrane : granules of endocrine and exocrine gland specific antibodies
- Immunohistochemical study of secretory granule membrane : 1. granules of intercalated duct and cell membrane specific antibodies
- Alpha-adrenoceptor gene variants and autonomic nervous system function in a young healthy Japanese population
- APPLICATION OF MONOCLONAL ANTIBODIES TO IMMUNOHISTOCHEMISTRY (Immuno-Histochemistry and Cytochemistry)
- IMMUNOHISTOCHEMICAL STUDY OF γ-GLUTAMYL TRANSPEPTIDASE DURING THE DEVELOPMENT AND MATURATION OF RAT KIDNEY
- Tbx5 and the retinotectum projection.
- ANATOMICAL DISTRIBUTION OF BIOTIN-BINDING PROTEIN/AVIDIN IN THE HEN OVIDUCT