末梢血幹細胞(CD 34陽性細胞)による血管再生治療(<特集>血管病変とアフェレシス)

概要

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Therapeutic angiogenesis using bone marrow cell transplantation has been reported, and this therapy was useful for arteriosclerosis obliterans (ASO). We attempted the same procedure using PBSCC. Five patients were included in this study, four were diabetic hemodialysis patients. They were all ASO patients : four were of lower limbs and one was of upper limb. The patients were given 5 μg/kg/day G-CSF for 4 days subcutaneously. PBSCC was done on day 4. For each patient, 8-10 L paeipheral blood was treated with an apheresis machine (CS-3000 or Spectra) and 1.3-9.9×10^7 CD 34 cells were harvested with mononuclear cells, red blood cells and platelets. We did not purify the CD 34 cells using the magnetic beads method and CD 34 enriched fluid (57-64ml) was injected at 60-130 points of limb muscle on the same day. Three of the five patients recovered. The disappearance of a foot ulcer continued for one year. But two patients had amputations below the knee because their ulcers were necrotic and infected. Therefore ASO of Fontaine 3 or 4 (not necrotic) was susceptible to this therapy.
日本アフェレシス学会の論文
2003-05-31