X-Ray Microanalysis at High Accelerating Voltage Detecting Aluminum Accumulation in Mouse Kidney after Short-Term Aluminum Administration

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Elemental analysis on Al was carried out by energy dispersive X-ray (EDX) microanalysis using high accelerating voltage at 300 kV with high permeability in thick samples at 1 μm thickness obtained from 27 ddY mice administered with Al for 1 to 17 weeks. The quantities of Al detected in the lysosomes of the convoluted tubule cells of the kidney did not relate to the Al administration period. A significant difference in the quantity of Al between the administration group and the non-administration group was found by stochastical analysis with two-way factorial analysis of variance followed by Fisher's PLSD for multi-comparison. However, the quantities of Al detected in the nuclei of the proximal convoluted tubule cells of the kidney suggested a correlation with the Al administration period. EDX microanalysis using thick sections and high accelerating voltage was useful in detecting the Al accumulated in the lysosomes and nuclei of the kidney cells. The EDX microanalysis was able to detect Al accumulated in the lysosomes and clarify the transitional changes of the quantities of Al detected during a short-term excessive Al administration.
日本組織細胞化学会の論文