LOCALIZATION OF ANTI-ALLERGIC AGENT IN RAT MAST CELLS DEMONSTRATED BY LIGHT AND ELECTRON MICROSCOPIC RADIOAUTOGRAPHY
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概要
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In order to demonstrate the intracellular localization of anti-allergic agent in rat mast cells, ^3H-labeled Tranilast, N-(3', 4'-dimethoxycinnamoyl) anthranilic acid was studied by both light and electron microscopic radioautography. The peritoneal mast cells of several Wistar strain rats were collected and incubated in vitro with Tranilast (100 μM) after sensitization with IgE and challenging with an antigen, demonstrating the inhibition of degranulation by type I allergic reaction. Then, the peritoneal mast cells were incubated in vitro with ^3H-Tranilast (100 μCi/ml) for varying time intervals from 0 to 60 min and the cell pellets were fixed, embedded and processed for light and electron microscopic radioautography. Light microscopic radioautograms revealed that silver grains were localized over the cytoplasm. Electron microscopic radioautograms revealed that silver grains were localized over and around the granules. The number of silver grains increased according to the prolongation of incubation time. Several rats were administered orally with ^3H-Tranilast (53 μCi/gm body weight). They were sacrificed at various time intervals and the back skins were taken out, fixed, embedded and processed for light and electron microscopic radioautography. Silver grains were observed to localize over the subcutaneous tissues, especially concentrating over the mast cells. From the results, it is concluded that ^3H-Tranilast was rapidly taken into mast cells in both in vitro and in vivo and it inhibited degranulation of type I allergic reaction.
- 日本組織細胞化学会の論文
著者
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Momose Yasunori
Toxicology Laboratories Kissei Pharmaceutical Co. Ltd.
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NAGATA Tetsuji
Department of Anatomy and Cell Biology, Shinshu University School of Medicine
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Nagata Tetsuji
Department Of Anatomy And Cell Biology Shinshu University School Of Medicine
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Nagata Tetsuji
Departmenrts Of Anatomy And Cell Biology Shinsyu University School Of Medicine
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Momose Yasunori
Department Of Clinical Pharmacy Faculty Of Pharmaceutical Sciences Toho University
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NISHIGAKI Toshiaki
Second Research Laboratory, Kissei Pharmaceutical Co. Ltd.
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NISHIGAKI TOSHIAKI
Department of Anatomy, Shinshu University School of Medicine
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Nishigaki Toshiaki
Second Research Laboratory Kissei Pharmaceutical Co. Ltd.
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MOMOSE Yasunori
Department of Anatomy, Shinshu University School of Medicine
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