血漿Renin活性測定法に関する検討 : 第1報 AngiotensinaseおよびReninの活性について

概要

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In 1940, a method for determination of retin from its enzymatic activity was published by Leloier et al. Thereafter, especially since 1963, several improvements for the indirrect assay of renin were made by many investigators. However, we frequently noted disagreement among the results obtained by these methods. In this paper, we described experimental results concerning the reliability of the rat bioassay for determination of angiotensin (AT) content as well as purification of renin from hog kidneys, properties and inhibition of angiotensinase (ATase) activity, and some properties of renin activity. Assay of AT was done by comparison of pressor response samples with standard AT (Hypertensin Ciba) solution when these solutions were injected to anesthetized rats intravenously. By this method, variance of the assay was ±7.2ng/ml (S.D.) when synthetic AT II were injected at a dose of 100ng/ml, ±4.7ng/ml at 50ng/ml and ±2.0ng/ml at 10ng/ml. The detectable lower limit was sometimes 1ng/ml, but usually 2-3ng/ml. We used the renin solution extracted from hog kidneys by the method of Haas et al. (Arch. Biochem. Biophys. 1965). At a pH of 7.5, we could not detect ATase activity in this solution. At pH=5.0, though the activity was detected to a certain degree, addition of diisopropylfluorophosphate (DFP) decreased the activity considerably.
社団法人日本泌尿器科学会の論文