Seasonal Variation of Paulownia Witches'-Broom Phytoplasma in Paulownia Trees and Distribution of the Disease in the Tohoku District of Japan

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概要

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• Seasonal variation of paulownia witches'-broom (PWB) phytoplasma within different organs (leaves, branch and trunk bark and roots) in paulownia trees was investigated by the amplification of a PWB-specific DNA fragment by the polymerase chain reaction (PCR). In leaf samples, PWB phytoplasma was first detected in June and the incidence gradually increased. On the other hand, the PWB was detected at relatively low incidence in branch bark, trunk bark and roots and the incidence did not change among seasons. A survey of PWB in 27 fields in the Tohoku district of Japan showed that malformed flower buds were observed in paulownia trees in almost all of the fields. PWB-phytoplasma was also detected by PCR from paulownia trees in almost all of the fields in Iwate and Fukushima Prefectures. The frequencies of trees in which phytoplasma was detected by PCR were higher than those in which symptoms were observed. These results indicated that PCR amplification of a PWB-specific DNA fragment is an effective tool for practical diagnose and that PWB is widely distributed in the Tohoku district of Japan.

• 一般社団法人日本森林学会の論文
• 1998-02-16

著者

• TAKAHASHI Tsuyoshi

  Faculty of Agriculture, Tokyo University of Agriculture and Technology

• YOSHIKAWA Nobuyuki

  Faculty of Engineering, Yokohama National University

• Kubono Takanori

  Tohoku Research Center Forestry And Forest Products Research Institute

• Yoshikawa Nobuyuki

  Faculty Of Engineering Yokohama National University

• Sahashi Norio

  Tohoku Research Center Forestry And Forest Products Research Institute

• Nakamura Hitoshi

  Faculty of Agriculture, Iwate University

• Ohgake Shigeto

  Faculty of Agriculture, Iwate University

• Ohgake Shigeto

  Faculty Of Agriculture Iwate University

• Yoshikawa Nobuyuki

  Faculty Of Agriculture Iwate University

• Nakamura Hitoshi

  Faculty Of Agriculture Iwate University:(present)institute Of Agriculture And Forestry University Of

• Takahashi Tsuyoshi

  Faculty Of Agriculture Iwate University

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