1. 乳酸脱水素酵素高温保存における賦形剤としての糖類の検討(平成14年度第48回低温生物工学会研究報告)

概要

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Effects of shelf temperatures on residual activities of lactate dehydrogenase (LDH) were determined. Raffinose, trehalose and mannitol were used as excipients. The mixtures of LDH, phosphate buffer and excipients were freeze-dried. They were stored at four shelf-temperatures (-25℃, ambient temperature, 50℃ and 70℃) from 0 to 30 days. From the results, raffinose and trehalose show good ability to stabilize activities of LDH. Although mannitol is a good excipient for short-term storage, but the residual activity of LDH was decreased drastically more than two weeks storage. Comparing a trehalose to raffinose, the trehalose samples show less heat-stability. Glass transition temperature of the excipients is the most important factor for stabilizing activity of LDH and other factors seem to concerned with the results.
低温生物工学会の論文
2002-12-28