アルギン酸カルシウムで固定したブタノール・イソプロパノール生産菌の連続発酵

概要

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To apply continuous fermentation by immobilized microorganisms in a 2-stage extraction butanol extractive fermentation process^<1)> previously reported, studies on the continuous fermentation of strain 172CY, a butanol-isopropanol-producing microorganism, immobilized by Ca-alginate were conducted. A down-flow column reactor with pulse flow inner circulation was designed for the continuous fermentation by the immobilized 172CY strain. The continuous fermentation proceeded stably, and butanol and isopropanol were produced relatively constantly for more than 30d under operation with the reactor. The production rate of butanol and isopropanol per unit volume of immobilized cells of the fermentation was expressed by a simple equation, that is the Mond equation multiplied by the individual fermentation inhibition of butanol and isopropanol, for the easy optimization of operation conditions. It was confirmed that the equation was also adaptable for the continuous fermentation applied in the 2-stage extraction butanol extractive fermentation process.^<1)>
社団法人日本生物工学会の論文
1993-01-25