移植を前提としたヒト培養組織生産に関する生物化学工学的研究

概要

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The in vitro cultures of human tissues have attracted a great deal of medical attention as a promising technique for repairing defective tissues in vivo. The 'Tissue Engineering' including a broad spectrum of technology platforms is defined as an integrated field based on the principles and methodologies of engineering and life sciences, and the development of biological substitutes to restore, maintain, or improve tissue function. The engineering problems have not been tackled so far although the studies on kinetic analysis, growth-model development, culture operation and bioreactor design should be required to reduce labor and time for in vitro production of cultured tissues with high quality. In manufacturing skin grafts, for instance, the raw materials of cells harvested from patients are heterogeneous, and the products of cultured tissues vary in the required size for individual epithelial sheets. Therefore, a reliable and robust process is desired for the production of cultured tissues with high reproducibility and quality. This review focuses on the strategies for developing the culture processes of keratinocytes targeting the epithelial sheet production, including (i) construction of kinetic model of cell growth, (ii) evaluation of cell properties based on image-analyzing techniques, and (iii) design of bioreactor system.
公益社団法人日本生物工学会の論文
2004-03-25