等濃度法を用いる吸光光度分析 : 原理と応用

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A new technique, the iso-concentration analysis, has been applied to the spectrophotometry. Two series of aliquots are taken. In the first series, each aliquot contains the same amounts of the substance x=(x, x, …), to be taken to which an incremental amount of the standards is added as x+y=(x+y_1, x+y_2, …). The second series contains an α times amount of the standards added to the first series, y'=(αy_1, αy_2, …). When all the aliquots of both series are made up to the same volume, V, the concentration of one of the aliquots in the first series is equal to that of second series. To all aliquots is added the constant amount of reagent which reacts with the species to be determined and forms the colored complex. The absorbance in any aliquot of the first and second series is given as Eqs. (1) and (2), respectively. A_<x+y>=εk_<x+y> (x+y)/V………(1) A_<y'>=εk_<y'> αy/V………(2) where A_<x+y> and A_<y'> are the absorbance. ε is the molar absorptivity, and k_<x+y> and k_<y'> are the constants. From both equations one obtains Eq. (3). αi=(k_<x+y>/k_<y'>) (1+(x/y)=(k_<x+y>/k_<y'>) (1+xz)………(3) where i=A_<x+y>/A_<y'>, and z=l/y. The plots of αi vs. z show an S-shape curve. All the curves of αi=f(z) have a common intersection at the point of k_<x+y>=k_<y'>, which shows i_k=1. One obtains Eq. (4). x=(α-1)/z_k=(α-1)y_k………(4) The intersection point can be obtained as the intersection of the curve of αi=f(z) with αi=α. If α=2, x is equal to y_k, i. e., the amount of substance present in the sample can be determined without using a calibration curve. The method was successfully applied to the determination of antimony using a reaction with Brilliant Green.
社団法人日本分析化学会の論文
1986-07-05

等濃度法を用いる吸光光度分析 : 原理と応用

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