THE DEVELOPMENT OF PHYTOMICROCHEMISTRY WITH SPECIAL REFERENCE TO CHROMATOGRAPHIC TECHNIQUES
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概要
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Classical phytomicrochemistry founded by H. Molisch using microscopic methods has been further developed by the author using modern chromatographic techniques. These include improved spot test analyses, paper chromatography, paper electrophoresis, thin layer chromatography, gas chromatography and high performance liquid chromatography. Among the new procedures in liquid chromatography, the use of a capacitance-conductance detector for weak electrolytes. and chelate derivatization of primary and secondary amines have played important roles. Increased versatility in the use of micro techniques can be expected from the methods of histochemical and enfleurage chromatographies applied to the analysis of living cellular tissue in situ. The former method involves the direct analysis of cell contents including oily substances, solutions and other visible material removed by a newly improved micromanipulator for appropriate analysis by chromatographic methods including GC/MS. The latter method was so named by reason of its similarity to a classical method used in perfumery in which volatile substances are absorbed on fresh fat and subsequently extracted by ethanol to prepare an "absolute". In the microanalytical adaptation, the fat is extracted with acetonitrile and the extract serves as a sample which is injected directly into the chromatograph or GC/MS combination without need for the removal of the solvent.
- 社団法人日本分析化学会の論文
- 1983-11-05
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関連論文
- ANALYSIS IN SITU OF CELL CONTENT BY MEANS OF AUTOMATIC HISTOCHEMICAL CHROMATOGRAPHY
- THE DEVELOPMENT OF PHYTOMICROCHEMISTRY WITH SPECIAL REFERENCE TO CHROMATOGRAPHIC TECHNIQUES
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- Evaluation of Crude Drugs by the Combination of Enfleurage and Chromatography (I) : Paeonol in Moutan Cortex and Paeoniae Radix
- Determination of Sweet Components in Stevia rebaudiana by High-Performance Liquid Chromatography : Ultraviolet Detection
- Studies on the Pharmaceutical Quality Evaluation of Crude Drug Preparations Used in Oriental Medicine "Kampo" VI. : ^C NMR Studies on Diberberine-monoglycyrrhizinate and Related Compounds
- Evaluation of Crude Drugs by a Combination of Enfleurage and Chromatography (IV) : On Flavor Components in Seeds of Amomum cardamomum and Elettaria cardamomum
- Studies on Pharmaceutical Quality Evaluation on Crude Drug Preparations Used in Oriental Medicine "Kampo" (V) : Physico-Chemical Properties of the Ion-Pair Compound of Berberine and Glycyrrhizin Formed in Kampo Decoctions Prepared from Glycyrrhiza Root an
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- Kinetic studies of fast equilibrium by means of high-performance liquid chromatography. III. Ternary complex formation between Ni(II) diethyldithiocarbamate and other unlike Ni(II) chelates.
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- Kinetic studies of fast equilibrium by means of high-performance liquid chromatography. VI. Separation of rotamers of formanilide.
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- Kinetic studies of fast equilibrium by means of high-performance liquid chromatography. X. Separation of rotamers of acetanilides.
- Kinetic studies of fast equilibrium by means of high-performance liquid chromatography. XIII. Separation of rotamers of N-alkyl-substituted amides.
- Kinetic studies of fast equilibrium by means of high-performance liquid chromatography. V. Stepwise complex formation of the nickel(II) ion with 2,2'-bipyridine and 1,10-phenanthroline.
- Kinetic studies of fast equilibrium by means of high-performance liquid chromatography. II. Ligand exchange of N,N-disubstituted dithiocarbamate chelates of Ni(II).
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