高速液体クロマトグラフィーによる化粧品中のイソプロピルメチルフェノールの分析

概要

論文の詳細を見る
High speed liquid chromatographic analysis of isopropylmethylphenol (3-methyl-4-isopropylphenol) on Nucleosil 5 NH_2 (5 μm. Macherey-Nagel) was studied. The condition was as follows; column size : 4 mm i.d. × 250 mm, detector : fluorescence spectrophotometer (exciting wavelength : 280 nm and fluorescence wavelength : 305 nm), eluent : 10% ethanol-hexane, flow rate : 1.16 ml/min, column temperature : 40℃. Determination was carried out successfully by using thymol or estradiol as an internal standard. The plots of peak height ratio vs. amount ratio (isopropylmethylphenol-thymol or estradiol) were linear between 2.5 ppm to 12.5 ppm. The experimentally prepared hair tonic and cream were diluted (1 : 100) with ethanol and dehydrated with anhydrous sodium sulfate. Thymol (5 ng/μl) or estradiol (20 ng/μl) was incorporated into this mixture as an internal standard. The averaged recoveries from hair tonic and cream ranged 100.5 % to 100.6 % and c. v. (%) were 1.25 to 1.02, respectively. This method is very simple, accurate and selective, therefore it is applicable to determine isopropylmethylphenol in cosmetic products at routin analyses.
社団法人日本分析化学会の論文
1980-04-05