液体クロマトグラフィーを用いる医療及び環境分析用超微量金属測定システムの開発

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The potential neurotoxicity of transferrin-bound aluminium has recently received increasing attention. A simple, sensitive, and selective method has been developed for the determination of transferrin-bound aluminium with 8-quinolinol by using affinity chromatography combined with kinetic differentiation mode HPLC. The most remarkable point of this method is that only transfertin-bound aluminiurn ion selectively response among many metal ions in human serum. The complex formation of released aluminium with 8-quinolinol is completed on the first column, which has an affinity to transferrin. The aluminium chelate is separated from the serum matrix on the second column, and detected at Ex 370 nm, Em 504 nm. It is well recognized that zinc is one of the important elements for human body. Although analytical applications of porphyrin as a highly sensitive spectrophotometric reagent for zinc have been investigated, the reaction of metal ion with porphyrin is generally very slow. In order to overcome this difficulty, several accelerators such as nucleoside were reported. In this study, it was found that zinc-TCPP (α, β, γ,δ-tetrakis (4-carboxyphenyl) porphine) complex is quantitatively formed within 20 min at room temperature in the presence of adenine. This effect has been successfully applied to the determination of zinc in human serum by HPLC. These proposed methods reported here will provide simple, rapid yet and sensitive techniques for trace elements of medical and environmental importance.
社団法人日本分析化学会の論文
2000-01-05

液体クロマトグラフィーを用いる医療及び環境分析用超微量金属測定システムの開発

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