原子吸光法による家と皮膚リンパ液中の銅,鉄及び亜鉛の定量

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A simple and precise methods for the determination of trace amounts of Cu,Fe, and Zn in lymph were investigated by graphite furnace and "one-drop method" in flame-atomic absorption spectrophotome-try (GF-AAS and OD-AAS). Lymph samples were collected from cutaneous leg lymphatic vessels of rabbits by cannulation, and diluted to appropriate concentration of each metal with O.1N-HN0_3 prior to measurements. Aliquots of 20μl for Cu and 100μl for Fe and Zn determination were then analyzed by GF-AAS(Cu) and OD-AAS (Fe and Zn). For example, the optimum operathg conditions of GF-AAS were as follows: wavelength: 324.8nm; drying: 15℃, ramp time: 40s, hold time: 20s; ashing I: 500℃, ramp time: 20s, hold time: 20s; ashing II: 950℃, ramp time: 15s, hold time: 20s; atomization: 2500℃, hold time: 6s; cleaning: 2600℃, ramp time: 1s, hold time: 3s. The calibration curves for Cu, Fe, and Zn were linear up to 60 ppb, 3.0ppm, and O.8ppm respectively. The detection limit (S/N=3) was 2 pg for Cu, 7ng for Fe, and 3ng for Zn. The recovery of Cu added to lymph samples averaged (98士3)% with a relative standard deviation (n=10) of 1.O% for 1.Ong of Cu. The both GF-AAS and OD-AAS methods were applied to the determination of Cu,Fe, and Zn in lymph samples. As the results, the average levels of lymph Cu,Fe, and Zn in 24 healthy rabbits were (O.16_6±O.09_9)(SD)ppm,(O.91_3±O.53_6)(SD)ppm, and (1.45±O.78_1)(SD)ppm, respectively.
社団法人日本分析化学会の論文
1983-03-05

原子吸光法による家と皮膚リンパ液中の銅,鉄及び亜鉛の定量

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