フレームレス-2チャンネル原子吸光法によるppbレベルの尿中クロムの定量

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Direct determination of chromium in urine at ppb level was investigated by means of flameless-dual channel atomic absorption spectrometry by using the carbon tube atomizer equipped with ramp modc (each stages) and flash mode (atomization stage). To establish the optimum conditions for the direct and sensitive determination, several parameters were examined systematically. The recommended procedure is as follows; An urine sample acidified with nitric acid (10- or 20-μl, containing up to 0.3 ng of chromium) is injected into the cavity of the carbon tube atomizer with micropipet. The sample is then dried, ashed and atomized by passing a high electric current through the atomizer for the measurement of the atomic absorption at 357.8 nm (Cr). Under the optimum conditions, the height of the absorption peak was directly proportional to the absolute amount of the chromium injected into the carbon tube (up to 0.3 ng). The calibration curve was prepared by standard addition of known amounts of chromium (III) to urine or synthetic urine sample. The relative standard deviation for the peak signals calculated from ten injections of urine (20-μl) was 4% and 3.3% at O.1 ng and O.2 ng of chromium, respectively. The interferences of the background absorptions which were due to the molecular absorption and light scattering could be corrected simultaneously by use of a 352.0 nm (Ne) or 364.3 nm (Ti) wavelength of the resonance line. Chromium concentration in urine from 167 subjects, determined by proposed method, have ranged from (0.20〜10.72) ppb, with a mean of 1.95 ppb. These results are similar to recent values obtained by other investigators. The proposed method offers sufficient sensitivity and accuracy for the direct determination of chromium in uman urine.
社団法人日本分析化学会の論文
1976-06-10

フレームレス-2チャンネル原子吸光法によるppbレベルの尿中クロムの定量

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