アノイリナーゼ菌(K・A)の代謝に関する研究 : (IX)菌の代謝に及ぼすイソニコチン酸ヒドラジド, シスチンビスニコチン酸ヒドラゾンの影響

概要

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It has been known that vitamin B_6 or α-ketoglutarate has a competitive action on the antibacterial activity of INAH. On the other hand, B. aneurinolyticus has a close relation with the metabolisms of α-ketoglutarate and glutamate in which vitamin B_6 is concerned as a cofactor. Therefore, studies were made on the effects of the substance and its derivative, cystine-INAH on the organism as a means for clarifying the mechanism of INAH action. Both of the substances showed no effect on the growth of the organism, whereas the formation of thiaminase II was remarkably inhibited. Cystine-INAH did not exert the same inhibitory effect on the vitamin B_6 relating enzyme systems as INAH. This fact suggests that the inhibition is due to the hydrazo-group of INAH. On the other hand, the isonicotinate residue may be attributed to the inhibition of the synthesizing system of saccharide from α-ketoglutarate, since cystine-INAH also inhibited the system. It was also found that cystine-INAH is toxic to the system utilizing glycerine for the sucrose synthesis. Therefore, it may be concluded that the reason why cystine-INAH has a similar antibacterial effect to INAH in spite of that the substance has no effect on the vitamin B_6-relating enzyme systems is the remarkable inhibition by the substance of the saccharide synthesis.
日本ビタミン学会の論文