ビタミンB_1類のホルマリン・アゾ反応について : (II)ビタミンB_1類の呈色物質について

概要

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It was proved by the spectrophotometry of colored substances eluated from paper partition chromatography that colored substances occurred in the formalin-azo test of thiamine were identical with those in that of 2-hydroxymethyl-thiamine (I), 2-methyl-4-amino-pyridyl-5-(4-methyl-5-β-hydroxyethyl)-thiazole (3) (II), ethylthiamine (III), butylthiamine (IV), acetylthiamine (V), 4-methyl-5-β-hydroxyethyl-thiazole iodethylate (VI), and 4,5-dimethyl-thiazole iodethylate (VII). Therefore, it was assumed that reaction mechanism of formalin-azo test of thiamine should be the same as that of Prebulda-McCollum reaction of thiamine. However, it was recognized that two kinds of colored substances were occurred from each of diacetylthiamine and dibenzoylthiamine. The chemical structure of these substances were not settled yet.
日本ビタミン学会の論文