Pseudomonas属菌の色素代謝に関する研究(第6報) : Pseudomonas aeruginosa T359株の培養物からAnthranilic acidの分離

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As part of the studies on the pigments produced by Pseudomonas aeruginosa T 359,paper chromatography of pyoluteorin (II), phenazine-1-carboxylic acid (III), oxychlororaphine (IV) and the culture broth of the micro-organism have been studied in the solvent systems of n-butanol-benzene-ammonium buffer (16 : 1 : 3) and n-butanol-methanol-ammonium buffer (16 : 15 : 3).Thus, the bouillon and the synthetic culture broth were found to give two unknown spots, A and B, together with the spots for II, III and IV on chromatogram, while the peptone broth showed four spots, A, B, C and D, without any indication of the presence of II, III and IV.From the synthetic culture broth, compound A was isolated and identified as anthranilic acid.
公益社団法人日本生物工学会の論文
1959-02-15