Pseudomonas属菌の色素代謝に関する研究 : (第8報) Pyoluteorinの生産条件ならびにそのタンク培養法による生産

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The concentration of pyoluterin (I), pyocyanine (II), phenazine-l-carboxylic adic (III), oxychlororaphine (IV) and anthranilic acid (V) produced by Pseudomonas aeruginosa T359 was determined spectrophotometrically with samples eluted from paper chromatograms, the optical density being measured at a wave length of 307 mμ for I and 336 mμ for V in ethanol-0.01 N HCl (10 : 1 v/v), 369 mμ for III and 368mμ for IV in chloroform, and 520mμ for II in 0.1 N HCl.The pyoluteorin formation by the organism was, then, studied in a synthetic medium containing 0.2% urea, 2.5% glycerol and various salts, The optimum salt concentrations were found to be 0.1〜0.4% KH_2PO_4,0.5〜1.0% CaCl_2・2H_2O, 0.0005〜0.001% FeSO_4・7H_2O and 0.05% MgSO_47H_2O. Among those ions, K^+, PO_4^<--->, ClFe^-, Fe^<++>, SO_4^<--> and Mg^<++> were indispensable and Ca^<++> served as an activator for the pyoluteorin formation. Carbonnitrogen ratio in a medium for the best pyoluteorin formation was 20 : 1. Addition of glycine, L-leucine, DL-isoleucine, DL-serine, DL-threonine, L-arginine or L-tryptophan increases the pyoluteorin formation, whereas L-methionine, L-cystine, L-histidine, L-glutamic acid, L-aspartic acid or L-proline inhibits the formation.Good yields of pyoluteorin were also obtained with the organism when a medium containing bouillon or corn strrp liquor as a sole nitrogen source and glycerol as a carbon source was used. Fermentation with the organism in a fifty-liter stainless steel fermenter constantly afforded a yield of two hundred and fifty milligrams or more per liter of pure pyoluteorin crystals.
公益社団法人日本生物工学会の論文
1960-01-15

Pseudomonas属菌の色素代謝に関する研究 : (第8報) Pyoluteorinの生産条件ならびにそのタンク培養法による生産

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