カップ法によるビタミンB群の微生物定量に関する基礎的研究 : (XVII)Saccharomyces carlsbergensisを用いる薄層カップ法によるビタミンB_6の定量

概要

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An agar-plate diffusion method for the quantitative determination of vitamin B_6 in natural materials using a thin-layer cup-plate containing Atkin's basal medium and 1% agar (0.75 mm thickness, pH 5〜6) was presented : On incubation at 30℃ for 12 to 16 hours, reproducible, clear and sharply-defined growth zones on the assay plate were steadily obtained and a linear response to pure vitamin B_6 solution containing 20 to 2,000 mμg per ml was demonstrated with the probable errors of 2 to 3% on statistical analysis. The total vitamin B_6 values in several natural materials obtained by thin-layer cup-plate assay were as equal as those obtained with turbidimetric ones. These results strongly suggest that the thin-layer cup-plate method using S. carlsbergensis can be used for the routine microbioassay of vitamin B_6 in natural products.
日本ビタミン学会の論文
1972-04-25