清酒酵母の生理 (第6報) : 好氣的條件に於ける琥珀酸の生成 (I)

概要

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KLEINZELLER (1941) has observed that the yeast can form succinic acid from the added glucose under aerobic conditions in remarkable quantities only in 0.5% bicarbonate solution, but not in phosphate buffer. From this observation, he raised an opinion against the KREBS cycle theory for succinic acid formation by the yeast. The authors recognized that, when Sake-yeast was used, succinic acid was aerobically formed not only in the bicarbonate solution, but also in the phosphate buffer from the added glucose, or from the intracellurar substance as substrate, and indeed, they could isolate 34 mg of succinic acid from the culture of Sake yeast (6.2g of dry weight) incubated aerobically at 30℃ for 3 hr in the phosphate buffer solution (pH 7.2) added with 5.4g of glucose, whereas 134 mg of the acid was isolated in the simultaneous presence of 1.7% glutamate.
社団法人日本生物工学会の論文
1952-11-15