HPLCを用いたウロカニン酸の定量法とヒト汗および皮膚におけるウロカニン酸含量

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A method of the high performance liquid chromatographic measurement of Z-urocanic acid (cis-form) and E-urocanic acid (trans-form) is described. The analytical method for the measurement of Z- and E-urocanic acid employed were a Tosoh ODS 80_<TM> (250mm×4.6mm I.D.) column eluted with 20 mM potassium dihydrogenphosphate containing 1 g/l of sodium heptanesulfonate (pH was adjusted to 3.7 by the addition of phosphoric acid): acetonitrile=93:7 (v/v) at a flow-rate of 1.0 ml/min. The UV detector was set at 264nm. The column temperature was maintained at 40℃. Under these conditions, Z-urocanic acid and E-urocanic acid were eluted at about 5.6 and 7.5 min, respectively. The detection limits of both compounds were 2 pmol (276pg) at a signal-to-noise ratio of 5:1. This technique was used to analyze the compounds in human sweat and on surface of human cheek and the total time required for one analysis was around 19 min.
1992-03-25