高速液体クロマトグラフィー用シス・トランスレチノール標準溶液の作製

概要

論文の詳細を見る
Since geometric isomers of retinol are not easily obtained, the individual determination of vitamin A groups in foods by high performance liquid chromatography has not yet been performed. In the present experiment, attempts were made to measure each isomer. The following procedure was found satisfactory for this determination. All-trans-retinal was transformed into a mixture of retinol cis-trans isomers by irradiation with visible light and reduction. The mixture was chromatographed on a silica gel column with authentic specimens (13-cis and all-tans forms). The concentration of each isomer was evaluated on the basis of the above results and the factors were confirmed in this work. Consequently, the mixture represents a retinol isomer standard. These factors were effective for isomer standardization by controlling the adsorptive activity of the silica gel, even when different columns are employed.
1992-10-25