高等植物のアスコルビン酸酸化酵素遺伝子の発現調節

概要

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Ascorbate oxidase is a copper-containing blue enzyme that occurs in plant tissues. Ascorbate oxidase expression in pumplin was studied. Ascorbate oxidase activity and protein rapidly increased during formation of pumpkin callus. Ascorbate oxidase was secreted into the culture medium in the cell suspension cultures. supporting the idea of cell wall localization of ascorbate oxidase. In addition, calcium joins markedly stimulated the secretion of ascorbate oxidase. A full-length c DNA clone for ascorbate oxidase was isolated. The amino acid sequence of pumpkin ascorbate oxidase is homologous to those of the other blue multicopper oxidases. Northern blot analyses showed that ascorbate oxidase was induced by auxin, suggesting that ascorbate oxidase is involved in cell growth. In pumplin callus, ascorbate oxidase activity and protein could be markedly increased by adding copper, a prosthetic metal of the enzyme. However, Northern blot analyses showed that ascorbate oxidase mRNA did not increase by adding copper. This suggests that copper controls ascorbate oxidase expression at translation or at a site after translation. A genomic clone for ascorbate oxidase was also isolated.
日本ビタミン学会の論文
1993-07-25