Trichoderma viride(reesei)変異株K-10-34の生産するキシナラーゼの固定化

概要

論文の詳細を見る
In order to make up a bioreactor system for the formation of xylobiose(X_2), a xylanase(TX)from Trichoderma viride(reesei)Mutant K-10-34 was immobilzed on DAIAION^<[○!R]> HP20(a nonpolar high-porous styren polymer), and further crosslinked with glutaraldehyde. For effective X_2 formation from the substrates by the immobilized HP20-TX, it was necessary to employ a lower size of carried(below 150 μm in diameter)and to wash with surfactants at regular intervals. Osavan^<[○!R]>(benzalkonium chloride solution)was a good disinfectant to prevent contamination of the packed-bed type reactor for continuous operation. On the other hand, soluble immobilized TXs such as glutaraldehyde-crosslinked TX and albumin-immobilized TX had better thermostability than that of native TX, and appeared to be well-suited for a membrane reactor system because of the superior recovery of the enzyme by ultrafiltration.
社団法人日本生物工学会の論文
1991-05-25