Trichoderma viride (reesei)変異株K-10-34のキシラナーゼ : 精製およびキシロビオース生成に関する性質

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A xylanase (TX) from a solid-state wheat bran culture of Mutant strain No. K-10-34 of Trichoderma viride (reesei) was purified by ethanol fractionation and CM-Sepharose CL-6B column chromatography. The purified TX was found to be homogenous on PAGE. The molecular weight was estimated to be 12,000 by Sephadex G-75 gel filtration, 19,000 by SDS-PAGE, 14,500 and 4,800 by 8 M Urea-SDS-PAGE. The isoelectric point was 9.1. The maximum xylobiose (X_2)-forming activity was obtained at pH 5.0 and 55℃. pH and thermal stable ranges were pH 3.0〜9.0 and up to 50℃. The purified TX could not hydrolyze X_2,but hydrolyzed xylotriose (X_3), xylotetraose (X_4) and xylopentaose (X_5). When X_3,X_4 and X_5 were used as substrate, X_2 was preferentially accumulated. In the final stage of the reaction, the hydrolysis products were X_2 and xylose (X_1), to reach the molar ratio of X_2 to X_1 to 3.
社団法人日本生物工学会の論文
1990-11-25