キシロビオース製造に有用なキシラナーゼを生産するChaetomium gracile変異株1161の造成

概要

論文の詳細を見る
Mutation experimetns were carried out to increase the high X_2-forming (X_2-Form) xylanase activity of Chaetomium gracile IFO 6568 (CG 6568) by irradiation with ^<60>Coγ-ray, followed by checking clear-cut zone formation on xylan-containing agar plates, xylan saccharifying(Xy-S)activity and xylan turbidity-decreasing(Xy-T)activity. The selected mutant No. 1161(Mutant 1161) was 115-fold better than the original strain CG 6568 in the X_2-Form. activity of the enzyme produced by solid state fermentation at 30℃ for 5 days.Strain CG 6568 produced three xylanases with low activity, but Mutant strain 1161 produced one xylanase with hyper activity and two xylanases each with low activity. The maximum X_2-Form. activity of crude xylanse for CG 6568 was at pH 6.5 and 60℃, but that of Mutant 1161 was at pH 5.0 and 50℃. The crude xylanse from mutant 1161 produced X_2 from birch wood xylan and malt cake xylan very efficiently.
社団法人日本生物工学会の論文
1992-01-25