"空蒸し"滅菌ふすま麹培養におよるChaetomium sp.のキシロビオース生成用酵素の生産

概要

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Industrial production of a xylobiose (X_2)-forming-enzyme from Chaetomium gracile 1161 in solid state fermentation by the non-watering "KARAMUSHI" sterilization system was studied. For seed production, submerged culture of mycelia was conducted with constarch-peptone (Sp) medium (for laboratory-flask scale) or 2-fold diluted Sp medium (for production-tank scale), because this strain does not sporulate much. Autoclaving at 120℃ for 20 min of non-watering wheat-bran (wb) 10 g in a 500 ml conical flask was the most suitable for high xylanase production. The effect of heating sterilization on wb medium was estimated by measuring the pH of wb extract. Scaling up in a pilot plant was done with 2.5 t of non-watering wb, autoclaved at 110℃ for 130 min, in 13 m^3 trommel followed by 5 days' aluminium-tray culture. The xylan-saccharifying (Xy-S) activity of 10-fold water extract of moldy-bran was 260 u/ml (19.5 u/ml as X_2-forming activity), and equivalent to about 90% of flask culture.
社団法人日本生物工学会の論文
1992-07-25