無機倍地におけるクロレラの増殖におよぼす水銀(II)イオンの毒性

概要

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The toxicity of Hg^<2+> for Chlorella sp. was studied using turbidimetry and viable cell counting. When the chlorella cells were incubated in 22 mM phosphate buffer containing Hg^<2+>, the viable cell number decreased with increasing amouts of mercury volatilized. Upon the addition of 0.5mg/l Hg^<2+> to the cell suspension (20mg/l), the viable cell number decreased from 2×10^6 to 8×10^2 cells/ml after 70 h of incubation. However, the turbidity of the cell suspension remained almost constant during the incubation period. Turbidity measurements showed a growth lag when the Chlorella cells pre-treated with Hg^<2+> were transferred into inorganic medium supplied with CO_2. Although the lag time was prolonged with increasing Hg^<2+> concentrations at pre-treatment, it was proved that the Chlorella cells grew immediately without lag by counting viable cells. The lag time observed turbidimetrically was found to be a function of the specific growth rate and the ratio of survival in the inoculum. The length of lag time measured agreed well that calculated from a derived equation using the specific growth rate (μ=3.24d^<-1> and initial viability (r°).
社団法人日本生物工学会の論文
1989-05-25