ホローファイバー透析器における酵素反応のシミュレーション

概要

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The performance of a hollow fiber membrane dialyzer (Dow hollow fiber beaker dialyzer b/HFD-1 with molecular weight cut-off of 5,000) as an immobilized enzyme reactor was investigated. The enzymatic reaction studied was the hydrolysis of benzoyl-L-tyrosine p-nitroanilide by α-chymotrypsin.Permeation rates of the substrate and the product (p-nitroaniline) across the fiber walls were measured first on order to estabilish basic parameters for the system. Permeability of the substrate was dependent on the concentration of both these components, while that of product was independent of the concentrations. Adsorption of the substrate and the product to the hollow fiber membrane and the polyurethane sealant was also found to be significant.A batch hollow fiber reactor, in which the enzyme solution was contained inside the fibers and the substrate solution was initially placed outside the fibers, and a recycle hollow fiber reactor, in which the enzyme solution was contained outside the fibers and the substrate solution was recirculated throhgh the fibers, were used for the enzymatic reaction. Values calculated from a simple mathematical model describing the behavior of these immobilized enzyme reactor systems were in good agreement with the experimental data.
社団法人日本生物工学会の論文